Plasmid

Part:BBa_K4941105:Design

Designed by: Xinyu Chen   Group: iGEM23_ZJFH-Nanjing   (2023-10-12)


pylxp-ylER-LhYida


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 6901
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3846
    Illegal PstI site found at 6901
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1788
    Illegal XhoI site found at 2186
    Illegal XhoI site found at 5318
    Illegal XhoI site found at 5351
    Illegal XhoI site found at 10444
    Illegal XhoI site found at 10473
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 6901
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 6901
    Illegal NgoMIV site found at 5506
    Illegal NgoMIV site found at 11330
    Illegal AgeI site found at 2665
    Illegal AgeI site found at 4224
    Illegal AgeI site found at 4448
    Illegal AgeI site found at 6276
    Illegal AgeI site found at 9859
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1634
    Illegal BsaI site found at 9038
    Illegal BsaI site found at 10615
    Illegal BsaI.rc site found at 1049
    Illegal BsaI.rc site found at 2988
    Illegal BsaI.rc site found at 4771
    Illegal BsaI.rc site found at 10530
    Illegal BsaI.rc site found at 10813
    Illegal SapI site found at 1936
    Illegal SapI.rc site found at 757
    Illegal SapI.rc site found at 10061


Design Notes

none


Source

yarrowia lipolytica

References